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A, intracellular dialysis of GDP-β-S, a non-hydrolysable analogue of GDP, failed to mimic the GTP-γ-S response. The pipette solution contained 1 mM GDP-β-S and 10 mM BAPTA. B, intracellular dialysis of another non-hydrolysable GTP analogue, GppNHP, also triggered slow Ca2+-independent exocytosis. The pipette solution contained 500 μM GppNHP and 10 mM BAPTA.
