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. 1998 Jun 23;95(13):7614–7619. doi: 10.1073/pnas.95.13.7614

Figure 4.

Figure 4

Regulation of PPARγ expression in THP-1 cells is mediated by the PPARγ1 and PPARγ3 promoters. (A) RNase protection analysis of PPARγ mRNA in TPA-treated THP-1 cells by using antisense mRNA probes that distinguish PPARγ1, PPARγ2, and PPARγ3 transcripts. The PPARγ2 probe is predicted to give protected fragments of 216 nt for PPARγ2 and a common fragment of 104 nt for PPARγ1 and PPARγ3. The PPARγ1 probe is predicted to give protected fragments of 218 nt for PPARγ1 and 174 nt for PPARγ3. (B) Expression of the PPARγ1 and PPARγ3 promoters in RAW 264.7 cells and regulation by 0.1 μM TPA. The PPARγ1-luciferase reporter gene contains 3.0 kb of 5′ flanking information, whereas the PPARγ3-luciferase reporter gene contains 800 bp of 5′ flanking information.