Figure 6. Evolution of Ba2+ block of current in S631A mutant channels during depolarizing steps.
Top panel, original recordings during 2 s depolarizations to 0 mV (left) and 30 mV (right) before and after superfusion with 500 μm Ba2+. Capacitive transients have been removed. Bottom, development of fractional block, (Ic–IBa)/Ic, in the presence of 500 μm Ba2+ in the same experiment, upon depolarization to the 6 voltage levels indicated. Similar results were obtained in 6 other oocytes.