Table 1.

Effect of ωCgtx GVIA, ωAga IVA, nifedipine and ryanodine on g_Cl,Ca in the presence of apamin

	Single action potential		Action potential train
	Current peak (nA)	Total charge (pC)	Current peak (nA)	Total charge (pC)
Apamin (n = 6)	−0.08 ± 0.03	−6.7 ± 3.7	−1.7 ± 0.4	−279 ± 69
Apamin +ωCgtx GVIA	−0.09 ± 0.04	−8.6 ± 5.1	−1.5 ± 0.4	−255 ± 80
Apamin (n = 10)	−0.11 ± 0.01	−8.4 ± 0.9	−2.0 ± 0.3	−334 ± 58
ZApamin +ωAga IVA	−0.06 ± 0.01 **	−3.5 ± 0.9 **	−1.2 ± 0.2 **	−165 ± 36 **
Apamin (n = 9)	−0.22 ± 0.06	−20.9 ± 7.6	−3.0 ± 0.6	−490 ± 119
Apamin + nifedipine	−0.09 ± 0.03 **	−6.6 ± 2.6 *	−1.5 ± 0.4 **	−184 ± 53 **
Apamin (n = 5)	−0.24 ± 0.05	−30.9 ± 7.9	−2.8 ± 0.9	−481 ± 158
Apamin + ryanodine	−0.09 ± 0.05 *	−7.6 ± 4.4 *	−2.2 ± 0.8 *	−319 ± 131 *

Tail currents after a single action potential or after a train of six action potentials at 40 Hz were recorded in voltage clamp at a holding potential of −55 mV. Inward Ca²⁺activated Cl⁻ currents were isolated by blocking g_K,Ca1 with 100 nm apamin. Changes after adding nifedipine (10 μm), ωCgtx GVIA (300 nm),ωAga IVA (40 nm) or ryanodine (20 μm) were compared using Student's paired t test

^*P < 0.05

^**P < 0.01.