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A, with 100 μm cAMP in the pipette filling solution, activation of INa elicited a Ca2+ influx signal which was blocked by 5 mm Ni2+. Note the increase in fluorescence in the right line-scan, which is due to elevated extracellular Ca2+ (10 mm). B, the Na+ current and the Ca2+ influx signal elicited in the presence of 1 μm isoproterenol were completely blocked by 20 μm TTX.
