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IL-2 selectively enhances tyrosine phosphorylation of fakB. A CD18-positive T cell line was incubated with medium or IL-2 (15 ng/ml) for 2 or 30 min. Lysates were immunoprecipitated with antibody against FAK (A) or fakB (B) and immunoblotted with anti-phosphotyrosine mAb or the antibody used for immunoprecipitation as indicated. Similar results were obtained in three independent experiments.
