Figure 3.

SERCA activity in embryonic chicken cardiac myocytes

Embryonic chicken cardiac myocytes were infected with Ad.Sr1 or Ad.Sr2a. A, rates of ⁴⁵Ca²⁺ uptake by SERCA (nmol Ca²⁺ per mg protein) in cell lysates. Inset, Western blot shows similar expression levels of SERCA1 and SERCA2a detected by the same anti-c-myc antibody. B, Ca²⁺-dependent ATPase activity (%) measured at varying pCa. •, endogenous activity in control cells; ▪, activity in cells overexpressing SERCA1; □, activity in cells overexpressing SERCA2a.