Figure 4.

Cytosolic Ca²⁺ transients in embryonic chicken and neonatal rat cardiac myocytes

Embryonic chicken and neonatal rat cardiac myocytes were infected with Ad.Sr1 or Ad.Sr2. Cytosolic Ca²⁺ transients were measured in single cells using fluo-4 (A-D) or fura-2 (E and F). Each trace represents the average of transients from 15–30 cells over 3–5 preparations. Note the different time scales of the displayed transients from embyronic chicken (left) and neonatal rat (right). Ca²⁺ concentrations in E and F were calculated from the fura-2 fluorescence at 380 and 358 nm excitation (see Methods).