Figure 1. Capacitative and non-capacitative cation entry pathways in human platelets.

A, fura-2-loaded human platelets were stimulated with TG (1 μm) and IONO (50 nM) in Ca²⁺-free HBS (100 μm EGTA added) and 5 min later thrombin (10 U ml⁻¹) was added (n = 5). B, cells were stimulated with TG and IONO or the vehicles alone (Control) in Ca²⁺-free HBS (100 μm EGTA added) and 3.5 min later SrCl₂ (300 μm) was added (n = 7). C, cells were stimulated with TG and IONO in Ca²⁺-free HBS (100 μm EGTA added); 3.5 min later SrCl₂ (300 μm) was added to initiate Sr²⁺ entry followed by thrombin (1 or 10 U ml⁻¹; n = 7). D, cells were stimulated with TG and IONO in Ca²⁺-free HBS (100 μm EGTA added); 3.5 min later CaCl₂ (300 μm) was added to initiate Ca²⁺ entry followed by thrombin (10 U ml⁻¹; n = 6).