Figure 6. Effect of PKC inhibition on non-capacitative Sr²⁺ entry in human platelets.

A, cells were pretreated for 5 min at 37 °C with 3 μm Ro-31-8220 or the vehicle (DMSO) alone (Control). The platelets were then treated with 1 μm PMA in HBS containing 1 mm Sr²⁺(n = 6). B, cells were pretreated for 5 min at 37 °C with 3 μm Ro-31-8220 or the vehicle (Control). The platelets were then stimulated with TG (1 μm) and IONO (50 nM) in Ca²⁺-free HBS (100 μm EGTA added) and 3.5 min later SrCl₂ (300 μm) was added followed by thrombin (10 U ml⁻¹; n = 6).