Figure 8. Thrombin-induced non-capacitative Ca²⁺ entry is independent of store depletion.

Fura-2-loaded human platelets were pretreated for 30 min at 37 °C with 20 μm Xest C (B-D) or the vehicle (DMSO) alone (A). In A and B, cells were then stimulated with TG (1 μm) and IONO (50 nM) in Ca²⁺-free HBS (100 μm EGTA added) and 3 min later CaCl₂ (final concentration, 200 μm) was added to initiate Ca²⁺ entry (n = 4). C, cells were stimulated with 10 U ml⁻¹ thrombin in Ca²⁺-free HBS (100 μm EGTA added) and 3.5 min later TG (1 μm) and IONO (50 nM) were added (n = 5). D, cells were stimulated with 10 U ml⁻¹ thrombin in the presence of 1 mm external Ca²⁺ (n = 6).