Table 1.
Cell cycle stage | 48 h with 10% FCS | 72 h with 10% FCS | 72 h with 0.1% FCS | *16 h with aphidicolin | 6 h release frome aphidicolin | 12 h release frome aphidicolin | 24 h release from aphidicolin |
---|---|---|---|---|---|---|---|
G0/G1 | 59 ± 0.5 | 65 ± 1.2 | 73 ± 1.1 | 88 ± 1.4 | 42 ± 1.0 | 46 ± 0.7 | 63 ± 2.8 |
S | 5 ± 0.5 | 7 ± 0.6 | 7 ± 0.7 | 6 ± 1.7 | 22 ± 0.7 | 9 ± 1.4 | 5 ± 0.7 |
G2/M | 36 ± 1.1 | 28 ± 0.1 | 20 ± 1.1 | 6 ± 2.0 | 36 ± 1.1 | 45 ± 0.7 | 33 ± 2.1 |
For synchronization, cultures were growth arrested by serum starvation for 2 days followed by μM aphidicolin for 16 h. Data represent means ±s.e.m. (n = 5).