Figure 1. Comparison of layer 2/3 pyramidal cell dendritic and axonal arbor in fluorescence and bright-field image after biocytin-HRP staining.
A, fluorescence image of a layer 2/3 pyramidal neuron using two-photon excitation imaging. The image is a patchwork of highlight projections through two stacks of 23 frames. The neuron was filled via a somatic patch pipette with the fluorescence indicator OGB-1 (200 μM). B, bright-field micrograph of the same neuron that was also filled during whole-cell recording with biocytin (3 mg ml−1) via the patch pipette, fixed and developed afterwards with biocytin-HRP conjugated to avidin.