Figure 3. Changes of perimembrane and global [Ca²⁺]_c during Ca²⁺ mobilization.

A, i and iv, grey-scale images show the fluorescence of Calcium Green-C₁₈ attached to permeabilized RBL-2H3 mast cells (left) and Calcium Green added to the medium (right). Increases of the fluorescence (ΔF) evoked by IP₃-induced Ca²⁺ release (ii and v) and a saturating dose of CaCl₂ (1.5 mm, high calcium, iii and vi) are shown in purple. B, time course of the [Ca²⁺]_pm responses evoked by IP₃ (12.5 μm), ionomycin (Iono, 10 μm) and Ca²⁺ (5 μm, 5Ca) are shown on the left. Time courses of [Ca²⁺]_c recorded simultaneously with [Ca²⁺]_m are shown on the right.