Figure 3.

Laser-manipulated embryos porated for exogenous FITC delivery using an average laser power of 45 mW. (a, b) Fluorescence and brightfield image of a post-laser manipulated 8-cell stage embryo. A total of 6 pores were made in 2 cells, 3 pores per blastomere, using a beam dwell time of 100 ms and 3 pulsing events of the galvo. (c, d) Fluorescence and brightfield image of a post-laser manipulated 4-cell stage embryo. Same number of pores were made in the blastomeres as indicated in (a, b). Beam dwell time was set to 100 ms and the galvo was pulsed once. Scale bars for (a, b, c, d) represent 200 μm.