Skip to main content
. 2008 Mar 25;5(3):e64. doi: 10.1371/journal.pmed.0050064

Figure 4. The Influence of Statins, FTI, and GGTI Inhibitors and siRNA on Antibody-Mediated CDC and/or CD20 Detection.

Figure 4

Raji cells were incubated with either diluent or 1 μM cerivastatin (A), 10 μM fluvastatin (B), or 10 μM atorvastatin (C) for 48 h. Then, 1 × 106/ml of cells were incubated with saturating amounts of FITC-conjugated anti-CD20 mAb (B9E9) or IgG1 (isotypic control) for 30 min at room temperature in the dark. Binding of mAb to Raji cells was measured with flow cytometry.

(D) Raji cells were transfected with siRNA targeting HMG-CoAR or irrelevant siRNA using OligofectAMINE. After 48 h, 1 × 106/ml of cells were labeled as described above.

(E) Daudi cells were incubated with either diluent or 10 mg/ml MβCD for 30 min. Then, 1 × 105/ml of cells were incubated with saturating amounts of the FITC-conjugated anti-CD20 mAbs ofatumumab, rituximab, B1, and B9E9 for 30 min at room temperature in the dark. The MFI corrected for the FITC–protein ratio was measured with flow cytometry.

(F) Ramos cells were incubated with either diluent or lovastatin (10 and 50 μM) for 48 h. Then, equal numbers of cells (1 × 105/well) were incubated for 60 min with 10 μg/ml alemtuzumab in the presence of 10% human AB serum as a complement source. Cell viability was measured in a MTT assay. The survival of cells is presented as percentage of corresponding diluent- or statin-pretreated cells without alemtuzumab.