Figure 2.

Enzyme activity of iNOS_FL and iNOS_8⁻9⁻ in transfected HEK 293 cells. (A) Nitrite accumulation (23 h) in the culture medium, after transfection with vector-only, iNOS_FL or iNOS_8⁻9⁻, is expressed as nanomoles per milligram of total cell protein. (B) NOS activity in lysates of transfected cells expressed as nanomoles of nitrite per milligram of total cell protein per 3 h. Data are means ± SD of three independent experiments, each done in duplicate. Invisible error bars are too small to be drawn.