Fig. 8. RNA interference phenotype of TgFCBP57 knockdown.

RNAi knockdown was done with dsRNA as described under “Experimental Procedures.” T. gondii cells, transfected with 1 or 2 μg of dsRNA, were used to infect human foreskin fibroblast cells, and at 18 and 24 h later, the cells were analyzed for TgFCBP57 by immunoblot. Results using antibody A (“Experimental Procedures”) are shown, although antibody B produced essentially the same result. [³H]Uracil incorporation of identical parallel cultures was measured and expressed as percentage of untreated (No RNA) value. Single-stranded sense RNA (sRNA,4 μg, 24 h) was used as another negative control. The numbers are average of three experiments with the mean ± S.E. as shown. Control Hsp90, detected by immunoblot (11), was unaffected (bottom panel).