Figure 1.

Effects of exposure to DETA-NO on cell respiration in J774 cells. Cells were incubated with different concentrations of DETA-NO (0.03 mM, triangles; 0.1 mM, circles; 0.5 mM, squares) and oxygen consumption was measured in samples taken at the indicated time points: before (open symbols) or after (filled symbols) addition of Hb (8 μM; n = 5). Oxygen consumption in DETA-NO-treated cells, here and in the subsequent figures, is reported as percentage of that observed in samples treated in the same way but without DETA-NO (controls). The rate of oxygen consumption in control cells was 14.3 ± 0.78 μM min⁻¹ (n = 5) and did not vary significantly throughout the experiment.