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. 1998 Jun 23;95(13):7631–7636. doi: 10.1073/pnas.95.13.7631

Figure 2.

Figure 2

Role of ONOO− and superoxide in the irreversible inhibition of respiration induced by DETA-NO. (A) J774 cells were treated with DETA-NO (0.5 mM) alone (squares) or in combination with the ONOO− scavenger methionine (20 mM, circles) or the superoxide dismutase mimetic MnTBAP (0.3 mM, triangles), and oxygen consumption was measured before (open symbols) or after (filled symbols) addition of Hb (8 μM, n = 4). (B) Cells of the J774 mutant C3C clone were treated with DETA-NO (0.5 mM) and oxygen consumption was measured before (open symbols) or after (filled symbols) addition of Hb (8 μM). The rate of oxygen consumption in control C3C cells was 11.8 ± 1.41 μM min−1 ( n = 3).