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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Methods. 2008 Feb;44(2):119–128. doi: 10.1016/j.ymeth.2007.10.009

Figure 4.

Figure 4

TLC-based assay to monitor AMP and aminoacyl-adenylate formation. A. Reaction time course showing chromatographic separation from top to bottom of Ala-[32P]AMP, [32P]AMP, and [32P]ATP. B. graphical representation of TLC data shown in panel A. Figure reproduced with permission from reference (51).