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Effect of PDT on protein expression of WAF1/CIP1/p21, cdk2, cdk6, cyclin E, and cyclin D1. The cells were subjected to PDT and harvested at 3, 6, and 12 hr post-PDT. Total cell lysates were prepared and 50–100 μg protein was subjected to SDS/PAGE followed by Western blot analysis and chemiluminescent detection. The details are described under Materials and Methods.
