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Effect of PDT on inter-binding between WAF1/CIP1/p21, cyclins, and cdks. The cells were subjected to PDT and harvested at designated times post-PDT. Total cell lysates were prepared, 50–100 μg protein were immunoprecipitated and subjected to SDS/PAGE and the binding with the other protein was measured by immunoblot analysis using antibodies directed against the other protein. The details are described in Materials and Methods.
