Table 1.

Primer sequences and PCR conditions for LOXL1 sequencing

Exon	Primer Sequence	MgCl2 (mM)	Annealing Temperature (°C)	Amplicon (bp)
1A	F: TCCCAGCCTGTTGCTTATTC	1.5	55	326
	R: AGGCCTGGTGGACAGAGAG
1B	F: AAGCAAGGAGCCTTCCTGTC	1.5	55	260
	R: GTACACCTGCCCGTTGTTCT
1C*	F: GCAGGTGTACAGCTTGCTCA	1.5	55	464
	R: ACACGAAACCCTGGTCGTAG
1D	F: CTCCTACCCGCAGCAGTTC	1.5	55	227
	R: GGTACTCGGGCAGCTCTTC
1E	F: CGACCAGGGTTTCGTGTACT	1.5	55	280
	R: AGGGTAGGCCTGCTCGAAG
1F	F: AGCAGGCCTACCCTGACC	1.5	55	340
	R: GCCTCCAGGAAGTTCTAAGGA
2	F: GGAGGTCTCTGGGCATTAGC	1.5	58	354
	R: TCACTGATGAAACGGTCAGG
3	F: TCTTCAGGGACAAGGAGTGG	1.5	58	357
	R: AGTGCTCATGGTGGGAGG
4	F: CAGGGAAGACTAGGCCCTCT	1.5	58	324
	R: CTGTGAGCAGAGCTGAGTGG
5	F: GAAACAAGCAGCATCACAGC	1.5	58	220
	R: GGCTGAAGCTTCTTTCAGGA
6	F: TCTGGTGAGCAGTTGAGGTG	1.5	58	202
	R: GGTGGGCAAACTCTTACTGG
7	F: CCCTCATTGACCCACTGTCT	1.5	58	356
	R: GCATGCAGAGCCACAGAGTA

Note.-F: forward; R: reverse.

*The SNP rs1048661 is located in exon 1 and was analyzed in this amplicon.