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Effect of phosphatase pre-treatment on kinase phosphorylation of proteins on microarrays. (a) CK2 substrates identified on phosphatase pre-treated high-content Human ProtoArray® 4. (b) Decrease in phosphate levels of protein microarrays treated with lambda protein phosphatase (LPP) followed by staining with Pro-Q Diamond. (c) Solution validation of CK2 substrates identified after LPP treatment.
