Table 2.
Primer list and amplicon lengths for 17 validated SFP-containing amplicons.
| Affymetix probe set | Primer Name | Forward primer (5' – 3') | Reverse Primer (5 – 3') | Predicted* amplicon size (bp) | Observed# amplicon size (bp) |
| Gma.7528.1.S1_s_at | GS01 | GTACCTTCCGGTGGATTCAA | TGGAATGATCACTTGGCAGA | 435 | 439 |
| GmaAffx.93250.1.S1_s_at | GS02 | AATCCGTGTGCCAGGAATAG | ACCCAATCAGACCAATGGAG | 416 | 420 |
| Gma.1164.2.S1_at | GS03 | GTCTTTTTGGCCTTGCATGT | TCTCAGCTTCTCCGTCCATT | 422 | 424 |
| Gma.1555.1.S1_a_at | GS04 | AACAACAGAGTGAGCCAGCA | ACCAGGGTACCTCCCTTGAC | 400 | 402 |
| Gma.1863.1.S1_at | GS07 | GCAGGAGTGCGTTTACCCTA | TGCTTTCACACCGCAAATTA | 417 | 424 |
| GmaAffx.86591.1.S1_at | GS08 | TGGGAGCTGTGTCAACAGAA | GGCCTCTCAAACTGAACGTC | 431 | 433 |
| Gma.4431.1.S1_at | GS12 | TAGCAGCCAGCCTGTGTATG | CGAAGGGTCCTAAACAACCA | 447 | 449 |
| Gma.8053.1.S1_at | GS16 | ATCTGAGGCAGCAGCAAAAG | TGCCATGGCCACTTTAGATT | 402 | 407 |
| GmaAffx.62051.1.S1_at | GS21 | GAAGCGTTGCATGCTTATCC | CATTCCAGTCACACCACCAG | 429 | 432 |
| GmaAffx.58155.1.S1_at | GS22 | GTTAAACGCACCGATGGACT | ACACACTCGCCAAACAATGA | 404 | 406 |
| Gma.5674.1.S1_a_at | GS23 | GCGGTGTTTCTTTCATGGTT | TCCCTCGTATATTCGGCATT | 429 | 433 |
| Gma.1449.1.S1_s_at | GS24 | GCCTTTCTTCAGTGGATTGG | TGATTCACAACCCCATTTGA | 442 | 444 |
| Gma.13293.1.S1_at | GS27 | TCTGCATTAAGCCACTGCAC | AATAGCAGCACCACGATTCC | 442 | 441 |
| GmaAffx.14067.1.S1_at | GS29 | GGCATCCCTCTCAAGAATGT | GCAACAAAAATGGGGTGAGA | 424 | 427 |
| GmaAffx.69322.1.S1_at | GS31 | AGTCTTATGTTGGCACAAAAACA | GCCAACTCTACCCACCAAGA | 433 | 433 |
| GmaAffx.28120.1.s1_at | GS36 | CATCAGACACAGACGGCACT | TCACACCAATCTCCCAAACA | 414 | 413 |
| GmaAffx.70836.1.S1_at | GS37 | CGTTCCAGTGGACATTATGC | AGATTCTTTTTGCCCAAGCA | 441 | 431 |
*Predicted size indicates the PCR product size anticipated by the primer3 program.
#Observed size indicates size of amplicon sequences after assembling the bidirectional sequence reads.