Table 3.
Library | Tissue | Total ESTs | Discarded ESTs | Analyzed ESTs | # GenBank | # EST in cluster | # cluster | # singletons | # consensus | % redundancy | % discovery |
K01 | Head | 35 | 9 | 26 | ES542703–ES542728 | 12 | 10 | 14 | 24 | 38.46 | 61.54 |
K05 | Head | 579 | 124 | 455 | ES542729–ES543183 | 225 | 108 | 230 | 338 | 35.16 | 64.84 |
K06 | Abdomen | 508 | 47 | 461 | ES543184–ES543644 | 250 | 110 | 211 | 321 | 42.73 | 57.27 |
K07 | Photophores | 506 | 52 | 454 | ES543645–ES544098 | 255 | 114 | 199 | 313 | 49.78 | 50.22 |
K09 | Thoracopods | 418 | 44 | 374 | ES544099–ES544472 | 227 | 108 | 147 | 255 | 55.61 | 44.39 |
Overall | 2046 | 276 | 1770 | 969 | 450 | 801 | 1251 | 44.35 | 55.65 |
Total ESTs = number of produced chromatograms; Discarded ESTs = number of low quality ESTs; Analyzed ESTs = number of sequences processed for clustering; # GenBank = accession numbers of ESTs deposited in GenBank; # EST in cluster = number of sequences in cluster; # clusters = total number of clusters; # singletons = number of putative transcripts identified by one EST; # consensus = number of non-redundant sequences; % redundancy = percentage of sequences not identified in an exclusive manner; % discovery = percentage of ESTs identifying putative new transcripts in the total EST analyzed.