Table 1.
CNS axon phenotypes in Rptp double and triple mutant embryos
| Phenotype (%) | ||||
|---|---|---|---|---|
| Genotype | n | Two | One/none | Cross-over |
| Ptp4E1 | 128 | 2 | 0 | 0 |
| Ptp10D1 | 128 | 1 | 0 | 0 |
| Ptp4E1 Ptp10D1; UAS-4E-GFP/elav | 124 | 7* | 0 | 0 |
| Ptp4E1 Ptp10D1 | 126 | 30† | 0 | 1 |
| Ptp4E1; Ptp69D1/Df(3L)8ex25 | 80 | 3 | 0 | 0 |
| Ptp10D1; Ptp69D1/Df(3L)8ex25 | 128 | 63 | 21 | 100 |
| Ptp4E1 Ptp10D1; Ptp69D1/Df(3L)8ex25 | 106 | 54 | 16 | 100 |
CNS longitudinal axons were scored by counting the number of longitudinal tracts in each hemisegment at the midpoint between commissures (visualized by residual 1D4 staining at stage 17). In wild-type embryos, there are three fascicles per hemisegment. n, total number of hemisegments (T2–A6) scored at stage 17. Two: hemisegments with only two longitudinal tracts where the outer bundle is missing or is fused to the medial fascicle. One/none: hemisegments with only one or no longitudinal tracts. Cross-over: segments where axons abnormally cross the midline. *Expression of UAS-4E-GFP in neurons using the elav-GAL4 driver produces incomplete rescue (from 30% to 7%, versus 1–2% in single mutants). However, the statistical difference between Ptp4E1 Ptp10D1 and Ptp4E1 Ptp10D1; UAS-4E-GFP/elav was highly significant (p < 0.0001, Chi-square test), indicating that pan-neural expression of Ptp4E rescues. † Statistical differences between Ptp4E1 Ptp10D1 and each of the single mutants were also significant (p < 0.0001, Chi-square test).