Figure 2.

The immunoreactivity of glycated LDL. The relative concentrations of LDL, LDL + Glu, and LDL + Glu + aminoguanidine (AG) that were required to obtain 50% of maximum binding (ED₅₀) in the solid-phase RIA. LDL from two subjects (A and B) were modified under the conditions described in the legend to Fig. 1. The ED₅₀ for LDL that had been incubated in the absence of glucose was normalized to a value of 1 for each mAb. The positions of the epitopes within apoB primary structure recognized by the 29 mAbs are shown (27) (C).