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. 1998 Jun 23;95(13):7643–7647. doi: 10.1073/pnas.95.13.7643

Figure 3.

Figure 3

Immunoreactivity of glycated LDL after SDS/PAGE. (A) Equal quantities of LDL (lane 1), LDL + Glu (lane 2), or LDL + Glu + aminoguanidine (AG) (lane 3), modified under the conditions described in the legend to Fig. 1, were subjected to SDS/PAGE and transferred to nitrocellulose membranes. Immunoreactivity of the transferred proteins was tested with the panel of anti-apoB mAbs and mAb 4G9 that is specific for AGE adducts. Results with three anti-apoB mAbs and mAb 4G9 are presented. (B) Western blot analysis of LDL (lane 1), reductively methylated LDL (lane 2), and LDL + Glu (lane 3) with an antiserum specific for apoB residues 3,352–3,371 and anti-apoB mAb 278.