Table 3. Antibody-based protein array analysis of fibroblast- or MSC-conditioned medium.
| human fibroblast | human MSC | mouse fibroblast | mouse MSCs | hypoxia/normoxia | ||
| Epithelial-neutrophil activating peptide | ENA-78 | + | + | N/A | N/A | NC |
| Granulocyte colony-stimulating factor | G-CSF | + | + | +/− | +/− | NC |
| Granulocyte-macrophage colony-stimulating factor | GM-CSF | +/− | +/− | + | + | NC |
| Growth-related oncogene | GRO | ++ | + | N/A | N/A | NC |
| Growth-related oncogene α, CXCL1 | GRO-α | +/− | +/− | + | +++ | NC |
| CCL1 | I-309 | + | + | N/A | N/A | NC |
| Interleukin-1α | IL-1α | ++ | ++ | N/A | N/A | NC |
| Interleukin-1β | IL-1β | + | + | N/A | N/A | NC |
| Interleukin-2 | IL-2 | + | + | +/− | +/− | NC |
| Interleukin-3 | IL-3 | + | + | +/− | +/− | NC |
| Interleukin-4 | IL-4 | + | + | + | + | NC |
| Interleukin-5 | IL-5 | +/− | +/− | +/− | +/− | NC |
| Interleukin-6 | IL-6 | ++++ | +++ | +++ | ++ | NC |
| Interleukin-7 | IL-7 | +/− | +/− | N/A | N/A | NC |
| Interleukin-8 | IL-8 | ++ | ++ | N/A | N/A | NC |
| Interleukin-10 | IL-10 | + | + | +/− | +/− | NC |
| Interleukin-12 | IL-12 | + | + | +/− | +/− | NC |
| Interleukin-13 | IL-13 | +/− | +/− | + | + | NC |
| Interleukin-15 | IL-15 | +/− | +/− | N/A | N/A | NC |
| Interferon γ | IFN-γ | +/− | +/− | +/− | +/− | NC |
| Monocyte chemoattractant protein-1, CCL2 | MCP-1 | ++ | ++ | +++ | +++ | NC |
| Monocyte chemoattractant protein-2, CCL8 | MCP-2 | +/− | +/− | N/A | N/A | NC |
| Monocyte chemoattractant protein-3, CCL7 | MCP-3 | +/− | +/− | N/A | N/A | NC |
| macrophage colony-stimulating factor | M-CSF | + | + | N/A | N/A | NC |
| Macrophage Derived Chemokine | MDC | + | + | N/A | N/A | NC |
| Monokine induced by IFN-Gamma, CXCL9 | MIG | +/− | +/− | N/A | N/A | NC |
| Macrophage inhibitory protein-1β, CCL4 | MIP-1β | + | ++ | N/A | N/A | NC |
| Macrophage inhibitory protein-1δ | MIP-1δ | + | + | N/A | N/A | NC |
| Regulated on activation normal T cell-expressed and secreted, CCL5 | RANTES | + | + | + | + | NC |
| Stem cell factor | SCF | + | + | +/− | +/− | NC |
| Stromal cell-derived factor 1 | SDF-1 | + | + | N/A | N/A | NC |
| Thymus- and activation-related chemokine, CCL17 | TARC | +/− | +/− | + | + | NC |
| Transforming Growth Factor-b1 | TGF-β1 | +/− | +/− | N/A | N/A | NC |
| Tumor necrosis factor-α | TNF-α | + | + | +/− | +/− | NC |
| Tumor necrosis factor-β | TNF-β | + | + | N/A | N/A | NC |
| Epidermal growth factor | EGF | +/− | + | N/A | N/A | NC |
| Insulin-like growth factor-1 | IGF-1 | + | ++ | N/A | N/A | NC |
| Angiopoietin | Ang | +++ | ++++ | N/A | N/A | up |
| Oncostatin M | OSM | + | ++ | N/A | N/A | up |
| Thrombopoietin | TPO | + | ++ | + | + | up |
| Vascular endothelial growth factor | VEGF | ++ | ++++ | ++ | +++ | up |
| Platelet derived growth factor-BB | PDGF-BB | +/− | + | N/A | N/A | up |
| Leptin | Leptin | +/− | + | +/− | +/− | up |
| Brain derived neurotrophic factor | BDNF | + | ++ | N/A | N/A | up |
| B Lymphocyte Chemoattractant, CXCL13 | BLC | +/− | +/− | N/A | N/A | NC |
| CCL23 | Ck β 8-1 | +/− | +/− | N/A | N/A | NC |
| Eotaxin | Eotaxin | +/− | +/− | +/− | +/− | NC |
| Eotaxin-2 | Eotaxin-2 | +/− | +/− | N/A | N/A | NC |
| Eotaxin-3 | Eotaxin-3 | + | + | N/A | N/A | down |
| Fibroblast growth factor-4 | FGF-4 | − | + | N/A | N/A | NC |
| Fibroblast growth factor-6 | FGF-6 | +/− | + | N/A | N/A | NC |
| Fibroblast growth factor-7 | FGF-7 | +/− | + | N/A | N/A | NC |
| Fibroblast growth factor-9 | FGF-9 | + | ++ | N/A | N/A | NC |
| FMS-related tyrosine kinase 3 ligand | Flt-3 ligand | +/− | + | N/A | N/A | NC |
| Fractalkine | Fractalkine | + | ++ | N/A | N/A | up |
| granulocyte chemotactic protein-2 | GCP-2 | +/− | +/− | N/A | N/A | NC |
| Glial cell line-derived neurotrophic factor | GDNF | + | ++ | N/A | N/A | NC |
| hepatocyte growth factor | HGF | ++ | +++ | N/A | N/A | up |
| IGF binding protein-1 | IGFBP-1 | +/− | + | N/A | N/A | NC |
| IGF binding protein-2 | IGFBP-2 | ++ | ++ | N/A | N/A | NC |
| IGF binding protein-3 | IGFBP-3 | + | ++ | N/A | N/A | NC |
| IGF binding protein-4 | IGFBP-4 | + | ++ | N/A | N/A | NC |
| interleukin 16 | IL-16 | +/− | +/− | N/A | N/A | NC |
| Interferon-inducible protein of 10 kDa, CXCL10 | IP-10 | + | + | N/A | N/A | NC |
| Leukaemia inhibitory factor | LIF | + | ++ | N/A | N/A | NC |
| Tumor necrosis factor (ligand) super family, member 14 | LIGHT | + | + | N/A | N/A | NC |
| CCL13 | MCP-4 | +/− | +/− | N/A | N/A | NC |
| Macrophage migration inhibitory factor | MIF | +/− | +/− | N/A | N/A | NC |
| macrophage inflammatory protein-3α | MIP-3α | +/− | + | N/A | N/A | NC |
| Neutrophil-activating peptide-2 | NAP-2 | + | ++ | N/A | N/A | up |
| Neurotrophin 3 | NT-3 | + | + | N/A | N/A | up |
| Neurotrophin 4 | NT-4 | + | + | N/A | N/A | up |
| Osteoprotegrin | OPTGRN | ++++ | ++ | N/A | N/A | NC |
| CCL18 | PARC | + | + | N/A | N/A | NC |
| Placenta growth factor | PIGF | ++ | ++ | N/A | N/A | NC |
| Transforming Growth Factor-b2 | TGF-β2 | ++ | + | N/A | N/A | NC |
| Transforming Growth Factor-b3 | TGF-β3 | ++ | + | N/A | N/A | NC |
| Metalloproteinase inhibitor-1 | TIMP-1 | ++++ | ++++ | + | + | NC |
| Metalloproteinase inhibitor-2 | TIMP-2 | ++++ | ++++ | N/A | N/A | NC |
| 6Ckine, CCL21 | 6Ckine | N/A | N/A | +/− | +/− | NC |
| Cutaneous T-cell-attracting chemokine, CCL27 | CTACK | N/A | N/A | + | + | NC |
| IL12p70 | IL12p70 | N/A | N/A | + | + | NC |
| Interleukin-17 | IL17 | N/A | N/A | +/− | +/− | NC |
| Monocyte chemoattractant protein-5, CCL12 | MCP-5 | N/A | N/A | + | ++ | NC |
| Macrophage inflammatory protein-1α | MIP-1α | N/A | N/A | + | + | NC |
| Macrophage inflammatory protein-2 | MIP-2 | N/A | N/A | + | +++ | NC |
| Macrophage inflammatory protein-3b | MIP-3b | N/A | N/A | + | + | NC |
| Soluble tumor necrosis factor receptor 1 | sTNF-R1 | N/A | N/A | ++ | +++ | NC |
Human or murine dermal fibroblast- or BM-MSC-conditioned medium under hypoxic conditions for 24 h was analyzed with antibody-based protein array. The intensity of each dot was measured. “−”, not detected; +/−, weakly detected; + ∼ ++++, intensity of positive detection; N/A, not tested; protein levels in medium under hypoxic conditions are indicated as up, down or NC (no change) compared to normoxic conditions.