Fig. 2.

OsPR10a promoter activity in response to SA: a schematic representation of the OsPR10a promoter in the reporter construct. b A transient assay showing the OsPR10a promoter in response to SA. OsPR10a:LUC was bombarded into rice leaves, which were then incubated in MS medium or MS medium containing 1 mM SA at 28°C for 24 h. Protein extracts were made by dissociation in passive lysis buffer as described in “Materials and methods”. Relative luciferase activity is the ratio of the value obtained with the SA-treated OsPR10a:LUC divided by the value obtained with the buffer-treated OsPR10a:LUC. Bars indicate the standard error of three replicates