Fig. 1.

Characterization of the PR1 gene family in rice plants. a Amino acid sequence alignment of 12 putative PR1 proteins from rice. The predicted mature proteins encoded by these genes were aligned by GENETICS 9.0 (Software Development Co., Tokyo). Because the N-termini of OsPR1#052, #051, and #121 are considerably different from those of the other 9 OsPR1s, these regions were eliminated from the figure and the conserved C-termini among the 12 OsPR1s were aligned. The acidic and basic PR1 proteins from Arabidopsis (AtPR1-like and AtPRB1) and tobacco (NtPR1a and NtPRB1b) were used as representatives of acidic and basic PR1 proteins, respectively. The conserved amino acid residues among the 16 proteins are marked with black circles. Identical amino acids are boxed. b Phylogenetic analysis of rice PR1 proteins based on the mature proteins using UPGMA method. The predicted isoelectric point (pI) for each mature protein is shown on the right. Acidic, basic, and neutral proteins are shown in red, blue, and green, respectively. Refer to the description in a for details