Fig. 2.

Response of OsPR1 genes to pathogen infection. a Blast fungus infection. A conidia suspension of blast fungus race 003 was spray-inoculated onto Nipponbare (compatible, upper panel), and IL7 (incompatible, lower panel). The inoculated fourth leaves were harvested at 0, 3, and 6 days post inoculation (dpi) for RNA extraction. To semi-quantify the transcript level, RNA samples were subjected to qPCR, and the relative expression levels compared with that of a control actin gene are shown. The transcript levels at time 0, mock-inoculated, and blast-fungus inoculated leaves were shown as white, gray, and black columns, respectively. Bars indicate mean ± SD. The up-regulated genes by blast fungus-infection are marked with arrowheads. Gray arrowheads in the lower panel indicate early responsive genes. b Bacterial blight infection. The fourth leaves of Nipponbare rice plants were inoculated with a suspension of Xoo for bacterial blight disease, and the leaves were harvested at 0, 4, and 8 dpi for RNA extraction. The transcript level was analyzed by qPCR. The relative expression levels compared with that of the control actin gene are shown. Transcript levels at time 0, mock-inoculated, and bacteria-blight inoculated leaves are shown as white, gray, and black columns, respectively. Xoo-upregulated and -downregulated genes are marked with forward and reverse arrowheads, respectively. Bars indicate mean ± SD