Fig. 5.

Response of OsPR1 genes to defense-signal compounds ACC, SA, and JA. Expression levels of each OsPR1 gene in fourth leaves at 24 h after treatment with defense-signal compounds were analyzed by qPCR. Relative expression levels compared with that of the control actin gene are shown. The ACC-, SA-, and JA-inducible genes are marked with gray, dark gray, and black arrowheads, respectively. For ACC or SA treatment, plastic pots with rice seedlings were dipped in solutions containing 1 mM ACC or 3 mM SA solution, and incubated for 24 h in the greenhouse. C1 control for ACC- and SA-treatment (leaves of water treated plant); For JA treatment, pots with seedlings each was put in an air-tight clear plastic box, and a cotton pad with volatile methyl jasmonate (MeJA) dissolved in EtOH was put at the corner of the box to give a final concentration of 100 μM, and incubated for 24 h. C2 control for JA-treatment (leaves of plant treated with 0 μM of JA), bars indicate mean ± SD