Fig. 7.

Histological analysis of OsPR1#074 gene expression in transgenic rice plants. a Schematic diagram showing the construct of the introduced gene. The promoter sequence of OsPR1#074 was used. b Localized GUS staining in healthy 5 day-old transgenic rice seedlings (a–e). Because similar expression profiles were obtained in three independent transgenic lines, the data from a representative line is shown. f unhulled rice. c GUS staining of 2 month-old transgenic rice plants. a Healthy roots, b roots at 2 days after wounding, c–e leaves at 2 days after wounding. d GUS staining assay of 4th leaves at 5 (a, c, d) or 11 (b) dpi. Black arrows indicate stained mesophyll tissue. Brown arrows indicate the necrotic regions