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. 2008 Feb 18;205(2):423–435. doi: 10.1084/jem.20071489

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Copyright © 2008, The Rockefeller University Press

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Figure 8. — Trk activation is decreased in the cerebellum of MCMV-infected mice. Each sample (lane) analyzed represented pooled cerebella from three mice from control and MCMV-infected mice (total six control and nine MCMV-infected cerebella). The cerebella were lysed and normalized for protein concentration. Equivalent amounts were subjected to SDS-PAGE, transferred to nitrocellulose membranes, and developed with antibodies that were reactive with total Trk B. Antibody binding was detected by ECL. The membrane was stripped and reprobed with antibodies specific for the activated form of Trk (phosphorylated at tyr 490). Note similar levels of Trk B expression but decreased phosphorylated (activated) Trk in cerebella lysates of infected mice.