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. 2008 Feb 18;205(2):315–322. doi: 10.1084/jem.20070763

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Copyright © 2008, The Rockefeller University Press

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Figure 1. — TLR triggering activates the PI3K pathway in human pDCs. Purified pDCs were cultured with (A) 1 μM CpG-C or (B) Flu (multiplicity of infection [MOI] = 2) for 20 or 90 min with or without the PI3K inhibitor LY at 1 μM. Cells were stained with anti–p-AKT, as specified in Materials and methods. Representative histograms of at least three separate experiments are shown.