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. 2008 Feb 18;205(2):315–322. doi: 10.1084/jem.20070763

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Copyright © 2008, The Rockefeller University Press

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Figure 4. — PI3K δ is essential for IFN-α production by pDCs in response to TLR stimulation. (A) Expression of class I A p110 (α, β, and δ) and class I B (γ) PI3K subunits in human pDCs. Purified pDCs were cultured for 6 h as indicated, and RNA was extracted and analyzed by quantitative PCR. Expression levels are expressed after normalization to β-actin. Data are shown as the mean with standard deviation from three independent donors. (B) Purified pDCs were cultured with 1 μM CpG-C, either alone or in combination with various concentrations of the PI3K inhibitors p110 γ AS 604850, p110 δ IC 87114, or LY for 16 h. IFN-α production was measured by ELISA. The mean of three independent donors is shown.