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. Author manuscript; available in PMC: 2008 Mar 21.
Published in final edited form as: J Biol Chem. 2006 Mar 9;281(24):16681–16690. doi: 10.1074/jbc.M600223200

FIGURE 2. GHS-R is a functional receptor on astrocytoma cells.

FIGURE 2

A, ghrelin induces membrane ruffling and actin polymerization in U-87 cells. Cells were fixed, permeabilized, and stained with Alexa Fluor 488-labeled phalloidin 20 min after ghrelin treatment (100 ng/ml). B, ghrelin treatment induces a time-dependent increase in PKC phosphorylation in U-87 cells. pPKC, phospho-PKC; T-PKC, total PKC. C, ghrelin elicits intracellular calcium release in control (C) scrambled siRNA-transfected U-87 cells, but not in cells subjected to GHS-R silencing by RNA interference. FL, fluorescence. D, ghrelin activates PKC via a GHS-R-dependent pathway. There was an increase in PKC phosphorylation 10 min after ghrelin treatment in cells transfected with control siRNA, but no induction in GHS-R siRNA-transfected U-87 cells.