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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: FEBS Lett. 2008 Feb 13;582(5):810–814. doi: 10.1016/j.febslet.2008.02.008

Fig. 4.

Fig. 4

HMGA1 binds to the hNOS promoter. The hNOS2 5′-flanking sequence between −3658 and −2944 is depicted, with 13 DNA sequences (underlined and numbered) containing AT-rich regions used as probes for protein-DNA binding assays. The bottom panels show protein-DNA binding reactions using these probes after radiolabeling, in the presence (+) or absence (−) of synthetic HMGA1 peptide (HMGA1(2/3), 500 ng). Reaction mixtures of the probes and HMGA1(2/3) were then subjected to electrophoresis. Potential HMGA1 binding sites are denoted by bold type. A vertical line in the right margin designates regions −3506/−3375 and −3348/−3119, which are deleted (Δ) and tested functionally in Figure 5.