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. Author manuscript; available in PMC: 2008 Mar 22.
Published in final edited form as: J Biol Chem. 2005 Nov 22;281(3):1489–1494. doi: 10.1074/jbc.M511154200

FIGURE 4. Purification of a chymase-generated NK4-like fragment of HGF.

FIGURE 4

This figure shows an HPLC chromatogram of unreduced fragments generated by incubation of HGF with human chymase. As shown in the inset, aliquots of major peaks 1 and 2 were subjected to non-reducing SDS-PAGE. Peak 1 contains a ∼45-kDa protein representing an NK4-like fragment generated by chymase. This purified protein was used to inhibit HGF scatter factor activity as in Fig. 5. Peak 2 contains the ∼30-kDa HGF β domain plus disulfide-linked α fragment remaining after removal of the NK4-like fragment from the native protein.