These micrographs show results of incubating MDCK cells with medium alone (A), intact HGF (B), human chymase-cleaved HGF (C), and intact HGF plus the purified NK4-like fragment generated by chymase (D). Note that HGF loosens cell clumps, elongates and scatters individual cells from sheet-like aggregates, and induces the formation of tube-like structures (inset). The NK4-like fragment opposes these effects.