. Author manuscript; available in PMC: 2008 Mar 22.

Published in final edited form as: Am J Physiol Cell Physiol. 2006 Jul 5;291(6):C1258–C1270. doi: 10.1152/ajpcell.00637.2005

Table 1. Mouse prostasin mutagenesis primers.

Mutation	Forward Primer	Reverse Primer
R322A	AGCCCCGAAATTGTTAGCGCCCGTACTTTTCCTGC	GCAGGAAAAGTACGGGCGCTAACAATTTCGGGGCT
S238A	CCAGGGTGACGCTGGGGGCCCAC	GTGGGCCCCCAGCGTCACCCTGG
S313N-S314L	CATCATCCTGTCTTCAACTTAGCGGCAGCCC	GGGCTGCCGCTAAGTTGAAGACAGGATGATG
K319A	GCGGCAGCCCCGGCATTGTTAAGGCCCGT	ACGGGCCTTAACAATGCCGGGGCTGCCGC
K319A-R322A	GCGGCAGCCCCGGCATTGTTAGCGCCCGT	ACGGGCGCTAACAATGCCGGGGCTGCCGC
S313N-S314L-R322A	Sequential use of S313N-S314L and R322A primers
S313N-S314L-A315P	GTCTTCAACTTACCGGCAGCCCCGA	TCGGGGCTGCCGGTAAGTTGAAGAC
S313N-S314L-A315P-R322A	Sequential use of S313N-S314L-A315P and R322A primers
S313N-S314L-A315P-K319A-R322A	Sequential use of S313N-S314L-A315P and R322A primers