Table 2.
Effects of compensatory mutations on the infectivity of SFV4 genome
| Alteration in the palmitoylation site of nsP1 | Compensatory mutation introduced | Full-length RNA infectivity (p.f.u. μg−1)* |
|---|---|---|
| None | None | 9.0×105 |
| None | P181Q | 7.8×105 |
| None | L234F | 1.3×106 |
| None | Q357L | 1.2×106 |
| None | M124V+A197D | 4.4×105 |
| None | ΔG224+T352S | 2.0×105 |
| ΔCCC (418–420) | L234F | 5.7×105 |
| Δ RSLTCCC (414–420) | L234F | 1.0×103 |
*Infectivity of full-length SFV genomes was measured by infectious centre assay. The numbers shown represent data from one of two experiments with similar results.