Table 1. Short tandem repeat loci and PCR primers employed in this study, and their size in base pairs (bp).
Locus | Genome location | Repeat size | PCR primers | Amplicon size | Repeat sequence | Translated sequence |
ML2172 | 2583816–2583839 | 3 bp | F: ATCAACGCTGCGGTTTCGCAG | 151 bp | AGT | (intergenic) |
R: ATATGCATGCCGGTGGTGTGCT | ||||||
ML2334 | 2785432–2785494 | 3 bp | F: CGTTGGGTTCGATCGAATCGA | 131 bp | TTC | (intergenic) |
R: GCACGCCGACGGGAATAAGT | ||||||
ML1505 | 1816857–1816892 | 6 bp | F: ATGGCAACCATCTGGACCTACT | 173 bp | GCACCT | Pro-Ala repeat |
R: GGCCGCCGAACTGATCAA | ||||||
ML1182 | 1381661–1381723 | 12 bp | F: GTCAATGAGGTCGGGTTAGC | 157 bp | GAGGTTGTTGAG | Glu-Val-Val-Glu repeat |
R: GCGGTACGACAGTAGCTTCC | ||||||
ML0058 | 73077–73139 | 21 bp | F: ATTACCAATTTGCAAGGAGTGCTCAGCT | 186 bp | CTTGATCAAGTTGATTCCTGG | (pseudogene) |
R: GAAATTTGGCGGCCATATGCC | ||||||
ML2469 | 2945487–2945555 | 23 bp | F: GTCGCCCGGATACTGTTAAA | 188 bp | ATAATACTGTAGTGAACGACATC | (intergenic) |
R: TAAATCCGCTCCCAAATCTT | ||||||
ML2418 | 2893248–2893761 | 25 bp | F: GTCGAGACGATCCTTGTTGC | 189 bp | AGAATTTACCGGCGTTCA*ATAAGAA | (pseudogene) |
R: TGCGCTCCTAGTTGAGCTTC |
*: single nucleotide mismatch in second copy