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. 2008 Apr;17(4):725–735. doi: 10.1110/ps.073355508

Figure 5.

Figure 5.

Enzyme activities (relative light units) for wild-type RLUC and selected mutants. (A) Polyacrylamide gel for the RLUC expression strains shown in B. (B) Values are in vivo luminescence activities from E. coli strain BL21 after induction of RLUC with IPTG. (C) Values are in vitro luminescence activities of purified proteins in the presence of coelenterazine substrate. Note the similarity between the activity profiles (B) in vivo and (C) in vitro.