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. Author manuscript; available in PMC: 2009 Apr 11.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Feb 6;368(3):508–514. doi: 10.1016/j.bbrc.2008.01.128

Figure 2.

Figure 2

Immunological identification of the ERM proteins and Hsc-70 in lens fiber cell triton-insoluble fractions. For an independent confirmation of the MALDI-TOF-TOF MS-based protein identification results, immunoblot analysis was carried out to identify specific proteins using polyclonal antibodies. Different amounts of lens fiber cell triton insoluble protein fractions obtained from both mouse and porcine were immnoblotted for ERM (panel A) and Hsc-70 (panel B) proteins using phospho-threonine-specific anti- ERM and anti-Hsc 70 antibodies, respectively.