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. Author manuscript; available in PMC: 2008 Mar 24.
Published in final edited form as: Biochemistry. 2007 Feb 21;46(11):3013–3022. doi: 10.1021/bi0619472

Fig. 8.

Fig. 8

Model for the role of the RNA binding site within the C-terminal domain (RBD) in enhancing duplex unwinding activity of CYT-19. A, CYT-19 binds with low specificity to structured RNA (blue cylinders, which depict the secondary structure elements of the Tetrahymena ribozyme) via its C-terminal domain and efficiently unwinds helices that are not packed tightly against the body of the structured RNA (the P1 duplex is shown as red and green strands). B, Enhanced unwinding activity by CYT-19 is also observed upon addition of a simple duplex to P1 (P2 is shown as a single blue cylinder), suggesting that the primary recognition by the C-terminal domain is for double-stranded RNA located adjacent to the helix to be unwound. See Discussion for further details.