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Recombinant wild-type and mutant D1-C/D12 heterodimers. Aliquots of the nickel-agarose preparations (containing 3 μg of the D1-C polypeptide) were analyzed by SDS-PAGE. The polypeptides were visualized by staining with Coomassie Blue dye. The positions and sizes (kDa) of marker polypeptides are indicated on the left. Alanine mutants are shown in A; conservative mutants are shown in B.
